ES Cell and Transgenics Facility

Overview of Services


This shared resource of the Koch Institute provides fee-for-service support to all MIT investigators who utilize novel mouse models to study human diseases such as cancer. Access is available to all members of the MIT community, to the extent permitted by available capacity. Priority access is given to KI members, NCI-funded research projects and other contributing user groups in recognition of funding support.


Facility Leadership


Technical Director

Aurora Burds Connor, Ph.D.

Ph: 617-452-3658

Project Coordinator

Noranne Enzer

Ph: 617-324-3911




Facility FAX: 617-324-3912


Location and hours of operation

Hours Location

Monday - Friday

8:00AM - 4:00PM

Excluding Holidays    

MIT Bldg 76 Rm 195

Kendall Square,

500 Main Street

Cambridge, MA

(See Map below for directions)

Links and Resources


  1. Core Facility Webpage:
  2. Swanson Biotechnology Center


Name Description Price
129S4/Jae Male    *
Assisted Work    *
C57BL6j Female    *
C57BL6j Male    *
CMV-Cre B6 Female    *
CMV-Cre B6 Male    *
Culture of ES cells and preparation for blastocyst injection    *
Expansion and Stocking an ES Line or Clone 

Cells are expanded optimally and 3-5 stock vials are frozen.

DNA will be prepared from a portion of cells for confirmation by the investigator.  Alternately, growing cells can be given to you for a western, nothern or other analysis.

FLP B6 Female    *
FLP B6 Male    *
Pathogen Tests 

ES cells imported from outside MIT will be grown in quarantine without antibiotics, prepared for IDEXX and tested for pathogens (DCM charge of $575, not included)

Prep & Ship 1-2 Mouse lines for Karyotyping    *
Prep & Ship Additional lines for Karyotyping (in same shipment)    *
Small Targeting, Picking up to 96 colonies    *
Standard Targeting, Picking up to 288 colonies    *
TPA-/- Female    *
TPA-/- Male    *
Training    *
Vial of p2 NeoR MEFs    *

Search available services: View: by category alphabetically
ES Cell Culture (5)
Gene Targeting in ES cells (2)
Mice: Cre (2)
Mice: FLP/Other (4)
Mice: Wild Type (3)
Request Cells (1)
Training (2)