ES Cell and Transgenics Facility

Overview of Services


This shared resource of the Koch Institute provides fee-for-service support to all MIT investigators who utilize novel mouse models to study human diseases such as cancer. Access is available to all members of the MIT community, to the extent permitted by available capacity. Priority access is given to KI members, NCI-funded research projects and other contributing user groups in recognition of funding support.


Facility Leadership


Technical Director

Aurora Burds Connor, Ph.D.

Ph: 617-452-3658


Project Coordinator

Noranne Enzer

Ph: 617-324-3911

Facility email:


Location and hours of operation

Hours Location

Monday - Friday

8:00AM - 4:00PM

Excluding Holidays    

MIT Bldg 76 Rm 195

Kendall Square,

500 Main Street

Cambridge, MA

(See Map below for directions)

Links and Resources


  1. Core Facility Webpage:
  2. Swanson Biotechnology Center


Name Description
129S4/Jae Male   
C57BL6j Female   
C57BL6j Male   
CMV-Cre B6 Female   
CMV-Cre B6 Male   
FLP B6 Female   
FLP B6 Male   
TPA-/- Female   
TPA-/- Male   
Culture of ES cells and preparation for blastocyst injection   
Pathogen Tests 

ES cells imported from outside MIT will be grown in quarantine without antibiotics, prepared for IDEXX and tested for pathogens (DCM charge of $575, not included)

Small Targeting, Picking up to 96 colonies   
Assisted Work   
Standard Targeting, Picking up to 288 colonies   
Expansion and Stocking an ES Line or Clone 

Cells are expanded optimally and 3-5 stock vials are frozen.

DNA will be prepared from a portion of cells for confirmation by the investigator.  Alternately, growing cells can be given to you for a western, nothern or other analysis.

Prep & Ship 1-2 Mouse lines for Karyotyping   
Prep & Ship Additional lines for Karyotyping (in same shipment)   
Vial of p2 NeoR MEFs   

Search available services: View: by category alphabetically
ES Cell Culture (5)
Gene Targeting in ES cells (2)
Mice: Cre (2)
Mice: FLP/Other (4)
Mice: Wild Type (3)
Request Cells (1)
Training (2)